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biotin anti human p2ry12  (Bioss)


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    Bioss biotin anti human p2ry12
    Expression of microglial markers in immortalized cells. a The expression of the microglial surface markers CD11b, CD14, and <t>P2RY12</t> is shown by fluorescence exposure imaging (magnification ×60) in a culture of human primary microglial cells. b GFAP antibody, used as negative control, stained human astrocytes (magnification 40X). Microglial cells cultured on glass coverslips were fixed, permeabilized, and incubated with the respective antibodies for 2 h followed by washing with DAPI-containing washing solution for nuclear staining
    Biotin Anti Human P2ry12, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin anti human p2ry12/product/Bioss
    Average 90 stars, based on 1 article reviews
    biotin anti human p2ry12 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system"

    Article Title: Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system

    Journal: Journal of Neurovirology

    doi: 10.1007/s13365-016-0499-3

    Expression of microglial markers in immortalized cells. a The expression of the microglial surface markers CD11b, CD14, and P2RY12 is shown by fluorescence exposure imaging (magnification ×60) in a culture of human primary microglial cells. b GFAP antibody, used as negative control, stained human astrocytes (magnification 40X). Microglial cells cultured on glass coverslips were fixed, permeabilized, and incubated with the respective antibodies for 2 h followed by washing with DAPI-containing washing solution for nuclear staining
    Figure Legend Snippet: Expression of microglial markers in immortalized cells. a The expression of the microglial surface markers CD11b, CD14, and P2RY12 is shown by fluorescence exposure imaging (magnification ×60) in a culture of human primary microglial cells. b GFAP antibody, used as negative control, stained human astrocytes (magnification 40X). Microglial cells cultured on glass coverslips were fixed, permeabilized, and incubated with the respective antibodies for 2 h followed by washing with DAPI-containing washing solution for nuclear staining

    Techniques Used: Expressing, Fluorescence, Imaging, Negative Control, Staining, Cell Culture, Incubation

    Surface expression of key markers of microglia. a Flow cytometry analysis was used to measure the surface expression of CD11b, CD14, CD68, CD16, CD32, CD64, CD163, P2RY12, and TGFβR on the representative immortalized cell line hμglia 1A1. In each experiment, 100,000 cells were resuspended in 1 mL of cold PBS in the presence of 0.5 μg of the antibody or isotype control for 20 min on ice. Appropriate secondary antibodies were used in the absence of fluorophore-conjugated primary antibody, and the cell-antibody complexes were centrifuged and resuspended in PBS. In each FACS profile, the gray distributions represent the proportion of cells bound to the isotype control, whereas the red distributions represent the proportions of cells bound by the target antibody. b Quantification of the abovementioned markers as well as GFAP, CD4, and CCR5 on the surface of primary human astrocytes and immortalized microglia, as indicated
    Figure Legend Snippet: Surface expression of key markers of microglia. a Flow cytometry analysis was used to measure the surface expression of CD11b, CD14, CD68, CD16, CD32, CD64, CD163, P2RY12, and TGFβR on the representative immortalized cell line hμglia 1A1. In each experiment, 100,000 cells were resuspended in 1 mL of cold PBS in the presence of 0.5 μg of the antibody or isotype control for 20 min on ice. Appropriate secondary antibodies were used in the absence of fluorophore-conjugated primary antibody, and the cell-antibody complexes were centrifuged and resuspended in PBS. In each FACS profile, the gray distributions represent the proportion of cells bound to the isotype control, whereas the red distributions represent the proportions of cells bound by the target antibody. b Quantification of the abovementioned markers as well as GFAP, CD4, and CCR5 on the surface of primary human astrocytes and immortalized microglia, as indicated

    Techniques Used: Expressing, Flow Cytometry

    Immortalized mouse microglia displays key features of primary microglial cells. a Representative clonal populations of muμglia cells, 3D3, 2G6, and 1F9, express CD11b and Iba1 as shown by the immunocytochemistry microphotographs. 100,000 cells/well were fixed, permeabilized, and blocked, before incubation with the primary antibodies against CD11b or Iba1 at a 1:500 dilution. Alexa secondary antibodies were added at a 1:500 dilution before mounting and imaging. b Constitutive expression of CD11b and Iba1 RNA was detected in immortalized microglia by quantitative PCR. 500,000 cells/well were lysed and subjected to RNA purification followed by cDNA synthesis prior to amplification. The RNA sample from clone 3D3 was used as a reference. c Constitutive expression of P2RY12 and TGFβR was measured by quantitative PCR
    Figure Legend Snippet: Immortalized mouse microglia displays key features of primary microglial cells. a Representative clonal populations of muμglia cells, 3D3, 2G6, and 1F9, express CD11b and Iba1 as shown by the immunocytochemistry microphotographs. 100,000 cells/well were fixed, permeabilized, and blocked, before incubation with the primary antibodies against CD11b or Iba1 at a 1:500 dilution. Alexa secondary antibodies were added at a 1:500 dilution before mounting and imaging. b Constitutive expression of CD11b and Iba1 RNA was detected in immortalized microglia by quantitative PCR. 500,000 cells/well were lysed and subjected to RNA purification followed by cDNA synthesis prior to amplification. The RNA sample from clone 3D3 was used as a reference. c Constitutive expression of P2RY12 and TGFβR was measured by quantitative PCR

    Techniques Used: Immunocytochemistry, Incubation, Imaging, Expressing, Real-time Polymerase Chain Reaction, Purification, Amplification



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